Dos métodos rápidos para la purificación de la L-Glicol deshidrogenasa (NAD+) de Enterobacter Aerogenes
- Carballo García, Francisco Javier
- Martín Sarmiento, Roberto
- González Prieto, Josefa
- Bernardo Álvarez, Ana
- Rodríguez Salido, J. C.
ISSN: 0373-1170
Ano de publicación: 1989
Ano: 35
Número: 35
Páxinas: 31-41
Tipo: Artigo
Outras publicacións en: Anales de la Facultad de Veterinaria de León
Resumo
Two new procedures to purify L-glycol dehydrogenase (NAD+) from Enterobacter aerogenes are proposed. Both of them give very pure preparations (s. a. = 31,5-34 I.U./ mg protein), with a high yield (42-49% of initial activity), avoid the use of fractionation techniques harmful to the enzyme and are also adventageous in requiring only 6-8 hours.