B220 expression as an immunological marker for differentiation of Feline Leukemia Virus carrying cats

  1. Isadora Duarte Santos Frota 1
  2. Jéssica de Oliveira Souza 1
  3. Fernanda de Oliveira Busato 1
  4. Cristina Abreu de Araujo 1
  5. Flávio Curbani 2
  6. David Olivieri 3
  7. Carlos Eduardo Tadokoro 1
  1. 1 Universidade Vila Velha. Brasil
  2. 2 Departamento de Tecnologia Industrial, Universidade Federal do Espírito Santo. Brasil
  3. 3 Escuela Superior de Ingeniería Informatica. Universidade de Vigo. España
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Revista:
Ciencia rural

ISSN: 0103-8478

Ano de publicación: 2020

Volume: 50

Número: 12

Tipo: Artigo

DOI: 10.1590/0103-8478CR20190467 DIALNET GOOGLE SCHOLAR lock_openAcceso aberto editor

Outras publicacións en: Ciencia rural

Resumo

Feline leukemia virus (FeLV) causes an infection in cats that, in some cases, can also be reported with other pathologies, such as infection with feline immunodeficiency virus (FIV), feline infectious peritonitis (FIP), and lymphoma. Although, a compromised immune response is reported in these animals, little is known about the immunological state of their cells. To shed some light in this area, we studied peripheral blood samples from both infected and non-infected cats with FeLV, with or without FIV, FIP, and lymphoma. We tested a panel of monoclonal antibodies (n=11) against mouse and human antigens and we reported that cat leukocytes can be stained with anti-mouse B220 monoclonal antibody; therefore, percentages of B cells were evaluated in different cat groups. Our results showed that cats with FeLV and FIP, or with leukemia, presented a large decrease in B220+ mononuclear cells. However, FeLV+ cats without clinical signs, or with unspecific clinical signs, had the same amount of B220+ mononuclear cells as healthy cats (control cats). Since the expression of B220 is exclusively restricted to the naïve B cell population, we inferred that the absence of these B cells in FeLV+ cats is related to other conditions that affect B cell numbers, such as viral infections and leukemias. Therefore, the amount of naïve B cells in peripheral blood (i.e., B220+ cells) can be used to identify FeLV+ cats concomitantly carrying FIP or leukemia, from FeLV+ cats with lymphoma or without any clinical signs

Fonte de datos: Dialnet