Microbial Communities as Affected by Clarithromycin Addition in Four Acid Soils (NW Iberian Peninsula)

  1. Laura Rodríguez-González 1
  2. Elena García-Campos 2
  3. Ángela Martín 3
  4. Montserrat Díaz-Raviña 3
  5. Manuel Arias Estévez 1
  6. David Fernández Calviño 1
  7. Vanesa Santás Miguel 1
  1. 1 Universidade de Vigo
    info

    Universidade de Vigo

    Vigo, España

    ROR https://ror.org/05rdf8595

  2. 2 Misión Biolóxica de Galicia (Santiago de Compostela)
  3. 3 Misión Biológica de Galicia (Santiago de Compostela)
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Revista:
Spanish Journal of Soil Science: SJSS

ISSN: 2253-6574

Ano de publicación: 2023

Volume: 13

Número: 1

Tipo: Artigo

DOI: 10.3389/SJSS.2023.11319 DIALNET GOOGLE SCHOLAR lock_openAcceso aberto editor

Outras publicacións en: Spanish Journal of Soil Science: SJSS

Resumo

A laboratory experiment was carried out to investigate the response of the microbial communities in acid agricultural soils located in the NW Iberian Peninsula to the presence of clarithromycin. Four soils, with different organic C content and similar pH, and seven different concentrations of clarithromycin (0.49, 1.95, 7.81, 31.25, 125, 500 and 2,000 mg kg−1 of soil) were used, and microbial estimates were made after 8 and 42 incubation days. The phospholipid fatty acids (PLFA) technique was used to estimate the total microbial biomass and biomass of specific microbial groups as well as the microbial community structure (PLFA pattern). The microbial biomass (total and specific groups) was different in the four studied soils, the lowest values being exhibited by soils with the lowest organic C. The antibiotic addition showed a positive effect on microbial biomass (total and specific groups), especially at the highest dose; the effect being similar or even more accentuated with time passed after the addition (42 days ≥8 days). Principal component analysis (PCA) of the PLFA data carried out with the whole data set showed that the main determining factors of the microbial structure followed the order: soil > time incubation ≥ antibiotic dose. When the PCA was performed individually for each incubation time, the results indicated that microbial communities of the four soils were different. Likewise, for each soil, different microbial communities were observed depending on antibiotic concentration. The microbial biomass and PLFA pattern data were coincidentally showing that the clarithromycin addition favored fungi and G− bacteria more that bacteria and G+ bacteria; the effect being dose-dependent. Our data (microbial biomass, PLFA pattern) also demonstrated that the effect of clarithromycin addition on microbial communities in these four acid agricultural soils persisted even after 42 incubation days.

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