Preparation and characterization of antigen loaded chitosan nanoparticles for immunization

  1. Prasanna, Maruthi
Supervised by:
  1. Noemi Stefania Csaba Director
  2. Cyrille Grandjean Director

Defence university: Universidade de Santiago de Compostela

Fecha de defensa: 11 September 2020

Committee:
  1. Antonio José Almeida Chair
  2. Maria Victoria Lozano Lopez Secretary
  3. Bernard Offmann Committee member
  4. María José Alonso Fernández Committee member
  5. Rosana Simón Vázquez Committee member

Type: Thesis

Abstract

The currently available pneumococcal vaccines are not effective in complete prevention of pneumococcal infections. To develop a better vaccine, firstly, we produced a glycoconjugate antigen based on common pneumococcal vaccine. Based on the positive immune responses generated by the glycoconjugate antigen in the mice, we further envisage that their combination with the nanotechnologies would generate a vaccine with enhanced potential. In this line, we utilized chitosan nanoparticles to encapsulate the glycoconjugate. Finally, the developed nanovaccines generated an enhanced immune response against both the protein and polysaccharide components when compared to the naked glycoconjugate.