Glucógeno sintasa del tejido del manto de "Mytilus galloprovincialis" Lamarck, 1819
- Yolanda Ruiz Muñoz
- Pilar Suárez Alonso
- Fuencisla San Juan Serrano
ISSN: 0074-0195
Ano de publicación: 2007
Volume: 23
Número: 1-4
Páxinas: 11-20
Tipo: Artigo
Outras publicacións en: Boletín. Instituto Español de Oceanografía
Resumo
Glycogen synthase (GS) from Mytilus galloprovincialis Lamarck, 1819 mantle tissue is primarily in G6P-dependent form, whose activity is four times that of the G6P-independent form. Both forms present higher activity to pH: 7, but the D-form mantains 60 % of its maximum activity at extreme pH values, whereas that of the I-form drops to 25 %. The optimum temperature is 30-35 ºC for both forms, but the I-form is more stable between 30 and 40 ºC. Kinetic data on both enzymatic forms indicate the existence of cooperative effects with regard to the substrate and effector. The I-form affinity constant for the substrate UDPG is 1.9-2.3 mM and that of the D-form is 0.9-1.3 mM. Moreover, the D-form shows an activation constant for the efector G6P of 5.2-5.6 mM.