Preparation and characterization of antigen loaded chitosan nanoparticles for immunization

  1. Prasanna, Maruthi
Dirixida por:
  1. Noemi Stefania Csaba Director
  2. Cyrille Grandjean Director

Universidade de defensa: Universidade de Santiago de Compostela

Fecha de defensa: 11 de setembro de 2020

Tribunal:
  1. Antonio José Almeida Presidente/a
  2. Maria Victoria Lozano Lopez Secretario/a
  3. Bernard Offmann Vogal
  4. María José Alonso Fernández Vogal
  5. Rosana Simón Vázquez Vogal

Tipo: Tese

Resumo

The currently available pneumococcal vaccines are not effective in complete prevention of pneumococcal infections. To develop a better vaccine, firstly, we produced a glycoconjugate antigen based on common pneumococcal vaccine. Based on the positive immune responses generated by the glycoconjugate antigen in the mice, we further envisage that their combination with the nanotechnologies would generate a vaccine with enhanced potential. In this line, we utilized chitosan nanoparticles to encapsulate the glycoconjugate. Finally, the developed nanovaccines generated an enhanced immune response against both the protein and polysaccharide components when compared to the naked glycoconjugate.